All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: UVSY-150 (for 150 μg)

Cat. No.: UVSY-1500 (for 1.5 mg)

The lyophilized version is also available by inquiry, which can be transported at room temperature.

We also offer DNA-free T4 UvsY Protein, which has undergone a rigorous E. coli DNA removal process.​

Description

The molecular weight of T4 UvsY protein is around 16KDa. This protein can stimulate the DNA-dependent ATPase activity of T4 UvsX recombinase, and lower the critical concentration of T4 UvsX recombinase that is required for activity, further promoting strand replacement. T4 UvsY protein efficiently promotes the T4 UvsX recombinase-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, resulting in the release of gp32 and the binding of T4 UvsX recombinase. T4 UvsY protein is free from detectable nuclease activities.

Component

T4 UvsY protein (1.5 μg/μl, glycerol free)

Application

• DNA isothermal amplification
• RPA amplification
• Genetic testing

Heat Inactivation

60°C, 10min.

Storage Buffer

50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.

Storage

The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.

Reagents for Recombinase Polymerase Amplification (RPA)

• T4 UvsX Recombinase
• T4 UvsY Protein
• T4 Gene 32 Protein
• Bsu DNA Polymerase
• Sau DNA Polymerase
• Exonuclease III
• Bst DNA/RNA Polymerase
• Endonuclease IV

Instruction: RPA Reaction System (for reference only)

RPA Lyophilized Kits

In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:

Gen-1

• RPA Basic Kit (Gen-1), 
• RPA RT Basic Kit (Gen-1), 
• RPA Fluorescent Kit (Gen-1), 
• RPA RT Fluorescent Kit (Gen-1)

Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)

• RPA Basic Kit (Gen-2), 
• RPA RT Basic Kit (Gen-2), 
• RPA Fluorescent Kit (Gen-2), 
• RPA RT Fluorescent Kit (Gen-2)

Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)

• RPA Basic Kit (Gen-3), 
• RPA RT Basic Kit (Gen-3), 
• RPA Fluorescent Kit (Gen-3), 
• RPA RT Fluorescent Kit (Gen-3)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory