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We are proud to introduce our groundbreaking RPA Multiplex Fluorescent Lyophilized Kit (Gen-4). This revolutionary exclusive multiplex Recombinase Polymerase Amplification (RPA) kit is capable of completing direct amplification of DNA/RNA within just 10 to 20 minutes.

Cat. No.: RIRF3-48 (for 48T)

Cat. No.: RIRF3-480 (for 480T)

Description

This product is the Gen-3 version of RPA (Recombinase Polymerase Amplification) nucleic acid amplification reagents, featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination. Supplied as a ready-to-use lyophilized reagent, it includes all components except primers, probes, Mg²⁺, and the template.

To use, simply dissolve the RPA lyophilized particles in nucleic acid release solution, add the template, primers, and Mg²⁺. The final reaction volume is 25 µL. Under constant temperature conditions of 39 to 42°C, the system specifically recognizes and amplifies nucleic acid fragments of the target sample. Detection and assessment can be performed in conjunction with gel electrophoresis, real-time fluorescence, or universal nucleic acid detection test strips.

Ideal for rapid RNA and DNA amplification and detection.

This product is also compatible with Lateral Flow Test Strips.

Composition

Reverse transcriptase, Recombinase, Polymerase, ATP, dUTP, dATP, dCTP, dGTP, sucrose-sucrose phosphorylase, UGI, buffer, poly A, MgOAc.

Nucleic acid release solution (Optional): UDG, Tris-OAc, Triton   X-100, EDTA, AFSE-15, TET

Features

• First introduction of the dUTP-UDG contamination prevention system in RPA
• Effectively removes aerosol contamination at 10⁶ copies/mL
• A perfect companion for POCT and CRISPR

Related:

• Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
• Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
• Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
• Gen-4: RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory