All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: EXN3-10k (for 10KU)

Cat. No.: EXN3GF-10k (for 10KU, glycerol free)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

The lyophilized Exonuclease III is also available by inquiry, which can be transported at room temperature.

Description

Exonuclease III was derived from E.coli, with 3 '- 5' exonuclease activity, acts on double-stranded DNA, and gradually cuts single nucleotides from the 3 'OH terminal direction. Each time the enzyme binds to the substrate for catalysis, only a few nucleotides are removed, resulting in progressive deletion within the DNA molecule group. 

The best substrate of this enzyme is the flat end or 5' protruding end double-stranded DNA molecule, but it can also act on the notch of double-stranded DNA, degrading DNA molecules from 3', and releasing 5' single nucleotides. For the 3' protruding end, especially those more than 4nt, it almost does not cut at all, nor can it cut the thiophosphoryl lipid bond. 

The activity of Exonuclease III depends partly on the helical structure and varies according to the sequence (C > A = T > G). In addition, Exonuclease III also has de-purine / pyrimidine endonuclease activity, RNaseH activity, and 3' phosphatase activity.

Applications

• Nondirectional nested deletion

• Preparation of single strand bottom template for dideoxy sequencing

• Preparation of strand-specific probes

• Site-directed mutagenesis

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 50 μl reaction system at 37°C for 30 minutes with 0.15 mM sonicated duplex [3H]-DNA.

Storage

The minimum shelf life is 3 years at -20°C. Avoid repeated freezing and thawing.

Reagents for Recombinase Polymerase Amplification (RPA)

• T4 UvsX Recombinase
• T4 UvsY Protein
• T4 Gene 32 Protein
• Bsu DNA Polymerase
• Sau DNA Polymerase
• Exonuclease III
• Bst DNA/RNA Polymerase
• Endonuclease IV

Instruction: RPA Reaction System (for reference only)

RPA Lyophilized Kits

In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:

Gen-1

• RPA Basic Kit (Gen-1), 
• RPA RT Basic Kit (Gen-1), 
• RPA Fluorescent Kit (Gen-1), 
• RPA RT Fluorescent Kit (Gen-1)

Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)

• RPA Basic Kit (Gen-2), 
• RPA RT Basic Kit (Gen-2), 
• RPA Fluorescent Kit (Gen-2), 
• RPA RT Fluorescent Kit (Gen-2)

Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)

• RPA Basic Kit (Gen-3), 
• RPA RT Basic Kit (Gen-3), 
• RPA Fluorescent Kit (Gen-3), 
• RPA RT Fluorescent Kit (Gen-3)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

Only for research and not intended for treatment of humans or animals

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