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Cat. No.: DFSAULF-250 (for 250U)

Cat. No.: DFSAULF-1250 (for 1250U)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

DNA-Free Sau DNA Polymerase (Large Fragment) is derived from the DNA polymerase (Sau) I gene of Staphylococcus aureus, which is obtained by cutting 1-293 AA at its N-terminal. This enzyme retains the 5’-3‘ polymerase activity of DNA polymerase but lacks 5‘ - 3’ and 3‘ - 5’ exonuclease activities, which can be used for recombinase amplification.

DNA-Free Sau DNA Polymerase (Large Fragment) is a DNA polymerase with strand displacement activity, which can be used for recombinase isothermal amplification. The protein-DNA complex formed by the combination of recombinant enzyme UvsX and primers can find homologous sequences in double-stranded DNA. Once the primer locates the homologous sequence, a strand displacement reaction will occur. Sau DNA polymerase starts DNA synthesis, and exponentially amplifies the target region on the template. The replaced DNA strand will bind to SSB to prevent further replacement.

Our DNA-Free Sau DNA Polymerase (Large Fragment), on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Sau DNA Polymerase (Large Fragment) particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes.

Applications

• Primer labeling
• Second strand cDNA synthesis
• DNA strand displacement synthesis
• Single dA tailing

Heat Inactivation

75°C, 20 min.

Unit Definition

One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

Reagents for Recombinase Polymerase Amplification (RPA)

• T4 UvsX Recombinase
• T4 UvsY Protein
• T4 Gene 32 Protein
• Bsu DNA Polymerase
• Sau DNA Polymerase
• Exonuclease III
• Bst DNA/RNA Polymerase
• Endonuclease IV

Instruction: RPA Reaction System (for reference only)

RPA Lyophilized Kits

In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:

Gen-1

• RPA Basic Kit (Gen-1), 
• RPA RT Basic Kit (Gen-1), 
• RPA Fluorescent Kit (Gen-1), 
• RPA RT Fluorescent Kit (Gen-1)

Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)

• RPA Basic Kit (Gen-2), 
• RPA RT Basic Kit (Gen-2), 
• RPA Fluorescent Kit (Gen-2), 
• RPA RT Fluorescent Kit (Gen-2)

Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)

• RPA Basic Kit (Gen-3), 
• RPA RT Basic Kit (Gen-3), 
• RPA Fluorescent Kit (Gen-3), 
• RPA RT Fluorescent Kit (Gen-3)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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