All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: BSTGF-16k (for 16KU)

Cat. No.: BSTLB (for Bst DNA/RNA Lyo Buffer)

Bst P DNA/RNA Polymerase is an upgraded version of Bst DNA/RNA Polymerase through enzyme electronic re-structure and evolution screening (in silico Design & in vitro Evolution), which is generally used for LAMP or RT-LAMP amplification of DNA or RNA. For more details, please check here: Bst P DNA/RNA Polymerase.

Description

At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase.

Bst DNA/RNA Polymerase is suitable for isothermal amplification reaction of both DNA and RNA templates, which can detect low-sensitivity nucleic acid templates with great efficiency and specificity. Besides, with a special preparation process, this enzyme has a fast amplification rate and high tolerance to impurity. This product has a high concentration (32U/μl) and is glycerol-free, which can be used to establish a freeze-drying system.

Strong Recognition Ability to dUTP

Bst DNA/RNA polymerase has a strong recognition ability to dUTP. The dTTP needed for amplification reaction can be completely replaced by dUTP, so the amplification products all contain dUTP. By adding our Heat-Labile Uracil DNA Glycosylase (HL-UDG), aerosol pollutants will be completely removed in the initial reaction stage. The HL-UDG can be later inactivated irreversibly within 3 min at 65°C, which not only eliminates the pollutants but also ensures the normal amplification of nucleic acid. Therefore, the false positive caused by aerosol pollution in the reaction can be greatly reduced.

Comparison

Storage Conditions

Store the components at -20°C. Avoid multiple freeze-thaw cycles.

Additional Notes

• We provide robust and reliable solutions for the study of various diseases (African Swine Fever, COVID-19, etc) based on Bst DNA/RNA Polymerase, please contact us or email tech@sbsbio.com for more details.
• For isothermal amplification of DNA only, please see Bst DNA Polymerase.
• For the lyophilized version, please see PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads.

Bst DNA/RNA Lyo Buffer (Optional)

Bst DNA/RNA Lyo Buffer is a special lyophilization buffer system for Bst DNA/RNA Polymerase with 2.5 × concentration, including reaction buffer salt, Mg²⁺ and lyophilization excipient. dNTP and Bst DNA/RNA Polymerase (glycerol-free) can be added directly when preparing the freeze-drying system. With this optimized buffer, there is no need to optimize the conditions of freeze-drying protective agent.

Note: Bst DNA/RNA Polymerase (glycerol-free) doesn't contain Bst DNA/RNA Lyo Buffer. If Bst DNA/RNA Lyo Buffer is required, please include it when placing the order.

Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021. Please visit: 

https://www.science.org/doi/10.1126/science.acx9701

Instruction: Protocol (Bst DNA/RNA Polymerase, glycerol-free), Protocol (Bst DNA/RNA Lyo Buffer)

Related: 

• Bst DNA Polymerase Large Fragment
• Bst DNA Polymerase (comparable to 2.0)
• Bst Polymerase (comparable to 3.0)
• Bst Polymerase (glycerol-free)
• Bst DNA/RNA Polymerase (with superior reverse transcription activity) 
• Bst DNA/RNA Polymerase (glycerol-free)
• Bst P DNA/RNA Polymerase (glycerol-free) (with superior reverse transcription activity & hot-start property) 

Customized Lyophilized Microbead Service

Looking for a personalized solution for your research needs? 

Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!

Viewpoint

Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.

SBS Genetech: Leading the Next Generation Isothermal Amplification Technology

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory